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The Maize TILLING Project has received support from
the NSF, USDA and members of the maize community. We
are currently being funded by NSF PGRP award #0604765.
MTP was initially funded by joint NSF
award to the Seattle TILLING project and Purdue
University that established a TILLING facility at Purdue
and also USDA-CSREES Award 03-35300-13236 to develop
the mutant populations.
EMS-mutant populations were generated to MTP and materials
were donated by the following lab groups:
- Nathan Springer
- Pat Schnable
- Gerry Neuffer
- Sarah Hake
- Tom Brutnel
- Cliff Weil
Listed below are links to TILLING-related publications
as of October 3, 2005. This list will be updated shortly.
TILLING Reviews
Comai L and Henikoff S.
TILLING: Practical single-nucleotide mutation discovery.
Plant J. 2006 45:684-94.
Abstract Full-text
version
Gilchrist EJ and Haughn GW. TILLING moves beyond
functional genomics into crop improvement. Curr Opin Plant Biol.
2005 Apr;8(2):211-5.
Abstract
PDF
(108k)
Slade AJ and Knauf VC. TILLING without a plough: a new
method with applications for reverse genetics. Transgenic Res.
2005 Apr;14(2):109-15.
Abstract
Henikoff S, Till BJ and Comai L. TILLING. Traditional
Mutagenesis Meets Functional Genomics.
Plant Physiol. 2004 May 21 [Epub ahead of
print]
Abstract
PDF
(126k)
Stemple, DL. TILLING - a high-throughput harvest for
functional genomics. Nat Rev Genet. 2004 5(2):145-50.
Abstract
Henikoff S and Comai L. Single-nucleotide
mutations for plant functional genomics. Ann Rev Plant
Biol 2003 54:375-401
Abstract
PDF
(771k)
Arabidopsis
TILLING Project
Till BJ, Reynolds SH, Greene EA, Codomo
CA, Enns LC, Johnson JE, Burtner C, Odden AR, Young
K, Taylor NE, Henikoff JG, Comai L, Henikoff S. Large-scale
discovery of induced point mutations with high-throughput
TILLING. Genome Res. 2003 Mar;13(3):524-530
Abstract
PDF
(526k)
Spectrum of TILLING Mutations
Greene
EA, Codomo, CA, Taylor, NE, Henikoff, JG, Till BJ, Reynolds,
SH, Enns, LC, Burtner, C, Johnson, JE, Odden, AR, Comai,
L, Henikoff S. Spectrum of chemically induced mutations
from a large-scale reverse-genetic screen in Arabidopsis.
Genetics 2003 Jun;164:731-740
Abstract
PDF (162k)
Ecotilling
Comai,
L, Young, K, Reynolds, SH, Codomo, C, Enns, L, Johnson,
J, Burtner, C, Henikoff, JG, Greene, EA, Till, BJ, and
Henikoff, S. Efficient discovery of nucleotide polymorphisms
in populations by ecotilling. Plant J. 2004 Mar;37:778-786
Abstract
PDF
(268k)
Liu K, Goodman M., Muse S., Smith
JJ, Buckler E., Doebley J. Genetic Structure and Diversity
Among Maize Inbred Lines as Inferred From DNA Microsatellites.
Genetics 2003. Dec;165: 2117-2128.
Abstract
PDF
(312k)
Maize
TILLING project
Till, B.
J., Reynolds, S. H., Weil, C. Springer, N., Burtner,
C., Young, K., Bowers, E., Codomo, C. A., Enns, L. C.,
Odden, A. R., Greene, E. A., Comai, L. and Henikoff,
S. Discovery of
induced point mutations in maize genes by TILLING.
BMC Plant Biol. 2004 Jul 28; 4:12
Abstract
Full
text version
TILLING for crop improvement
Slade AJ,
Fuerstenberg SI, Loeffler D, Steine MN and Facciotti
D (2004) A reverse genetic, nontransgenic approach to
wheat crop imporvement by TILLING. Nat. Biotech. doi:10.1038/nbt1043.
PDF (400k)
TILLING protocols
Till, BJ,
Colbert, T, Tompa, R, Enns, L, Codomo, C, Johnson, J,
Reynolds, SH, Henikoff, JG, Greene, EA, Steine, MN,
Comai, L, and Henikoff, S. (2003) High-throughput TILLING
for functional genomics, in Plant Functional Genomics:
Methods and Protocols, ed. Grotewald, E. Humana Press,
236:205-220
Humana Press
Nucleases
for mismatch cleavage
Till, BJ,
Burtner, C, Comai, L and Henikoff, S. Mismatch cleavage
by single-strand specific nucleases. Nucleic Acids Res.
2004;32(8):2632-2641.
Abstract
Full-text
version
GelBuddy
Zerr, T
and Henikoff, S. Automated band mapping in electrophoretic
gel images using background information. Nucleic Acids
Res. 2005;33(9):2806-2812.
Full-text
version
High-throughput TILLING
Colbert
T, Till BJ, Tompa R, Reynolds S, Steine MN, Yeung AT,
McCallum CM, Comai L, Henikoff S. High-throughput screening
for induced point mutations. Plant Physiology. 126(2):
480-4, Jun 2001
Abstract
PDF
(846k)
PARSESNP: A webtool for
analyzing SNP and TILLING data
Taylor
NE, Greene EA. PARSESNP: A tool for the analysis of
nucleotide polymorphisms. Nucl. Acids Res. 31:3808-3811,
2003
Abstract
PDF (305k)
SIFT: A webtool
for predicting deleterious missense changes
Ng PC,
Henikoff S. SIFT: predicting amino acid changes that
affect protein function. Nucl. Acids Res., 31:3812-3814,
2003
Abstract
PDF (309k)
Choosing optimal regions for TILLING
McCallum
CM, Comai L, Greene EA, Henikoff S. Targeting Induced
Local Lesions in Genomes (TILLING) for plant functional
genomics. Plant Phys. 123(2): 439-442, Jun 2000
Abstract
PDF
(154k)
Targeting
Induced Local Lesions IN Genomes (TILLING)
McCallum
CM, Comai L, Greene EA, Henikoff S. Targeted screening
for induced mutations. Nature Biotechnology. 18(4):
455-7, Apr 2000
Abstract
PDF
(207k)
TILLING soybeans
Nielsen,
N, Ritchie, R. TILLING genes to improve soybeans. Agricultural
Reserch. July, 2005.
Full-text version
TILLING flies
Winkler
S, Schwabedissen A, Backasch D, Bokel C, Seidel C, Bonisch
S, Furthauer M, Kuhrs A, Cobreros L, Brand M, Gonzalez-Gaitan
M. Target-selected mutant screen by TILLING in Drosophila.
Genome Res. 2005 May;15(5):718-23.
Abstract
TILLING
worms
Cuppen
E, Vastenhouw N, Gort E, Mudde J, Plasterk R. A new
powerful approach for reverse genetics in C. elegans.
14th Bienniel International C. elegans Conference, June
29, 2003
Abstract (4k)
TILLING rats
Smits BM,
Mudde J, Cuppen E, Plasterk RH. Target-selected mutagenesis
of the rat. Genomics. 2004 Feb; 83(2): 332-4
Abstract
TILLING zebrafish
Draper
BW, McCallum CM, Stout JL, Slade AJ, Moens, CB. A high-throughput
method for identifying N-ethyl-N-nitrosourea (ENU)-induced
point mutations in zebrafish. Methods Cell Biol. 2004
77:91-112.
Pubmed
Wienholds
E, Van Eeden F, Kosters M, Mudde J, Plasterk RH, Cuppen
E. Efficient target-selected mutagenesis in zebrafish.
Genome Res. 2003 Dec;13(12):2700-7.
Abstract
(4k)
Hurlstone
AF, Haramis AP, Wienholds E, Begthel H, Korving J, Van
Eeden F, Cuppen E, Zivkovic D, Plasterk RH, Clevers
H. The Wnt/beta-catenin pathway regulates cardiac valve
formation. Nature. 2003 Oct 9;425(6958):633-7
Abstract
(4k)
TILLING a preselected
population of mutants
Perry JA,
Wang, TL, Welham TJ, Gardner S, Pike JM, Yoshida S,
Parniske M. A TILLING reverse genetics tool and a web-accessible
collection of mutants of the legume Lotus japonica.
Plant Phys. 131: 866-71, Mar 2003
PubMed
TILLING
detection using DHPLC and CEL I
Caldwell
DG, McCallum N, Shaw P, Muehlbauer GJ, Marshall DF,
Waugh R.A structured mutant population
for forward and reverse genetics in Barley (Hordeum
vulgare L.). Plant J. 2004 Oct;40(1):143-50
PubMed
TILLING e-brochure
(Li-cor, Inc.)
Automated
Reverse Genetics and the Li-cor 4300 DNA analysis system
What is TILLING?
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