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Fee:
The current cost for
a TILLING order is $750, but the price will increase
to $1500 on January 1, 2007. The Maize TILLING Project
must be a full-cost recovery service by July 2008 [link
to Cliff's letter]. The total cost of a TILLING screen
including labor and supplies is ~ $3000. Funding from
NSF will defray some of the TILLING costs as we transition
to "self-sufficiency".
Invoices for TILLING requests will be sent out on or
about the 21st of the month. When you submit your TILLING
order, you can include the PO number if that will be
the payment method. Payment by other means is to be
remitted to Purdue's Centralized Accounts Receivable
System (CARS) according to the directions on the invoice.
We will order the unlabeled primers for your request
when you submit the order, however, the IRD labeled
oligos will not be ordered until payment has been received.
If you have questions regarding the invoice, please
email: tilling-orders@lists.purdue.edu.
Primer Pre-screen Failures:
Users will have four
attempts to find a primer set that works for their target.
To date, only four of our 119 requests have required
three or more attempts to find suitable TILLING primers.
People who have tested out primers sets in their lab
prior to submitting the TILLING request usually pass
the pre-screen step the first time.
TILLING Failures:
Since we began taking public orders in December 2004,
we have had only two targets that we could not TILL.
TILLING failures will be handled on a case-by-case basis.
Completed Screens:
TILLING screens are considered
complete when we deliver an allelic series where there
is a 95% confidence level that at least one of the mutations
will be damaging (see "Criteria
for delivering an allelic series"). Until we
have a population with a higher mutation density, MTP
is not enforcing the "lowest quartile" provision
described on the STP site. However, we strongly encourage
users to select a target in which at least 25% of the
expected mutations will be non-silent.
Data Release:
The user has a 180-day
confidentiality period that begins once s/he is notified
by email that there are TILLING mutations for the gene
target. After 180 days, the TILLING data but not the
requestor's name, will be posted to the following databases
The "Maize TILLING Data"
section has more details regarding the information found
at each site.
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